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PeproTech all cytokines
a Ncr1 +/+ ILC1s and Ncr1 gfp/gfp ILC1s sorted from the liver of Ncr1 +/+ and Ncr1 gfp/gfp mice, respectively, were cultured with or without anti-NKp46 antibody (5 μg/ml) for 12 h. Representative flow cytometry (left) and statistics (right) of the IFN-γ + (top) and TNF + (bottom) of ILC1s ( n = 6). b Ncr1 +/+ ILC1s and Ncr1 gfp/gfp ILC1s sorted from the liver of Ncr1 +/+ and Ncr1 gfp/gfp mice, respectively, were cultured with or without C1498 AML cells expressing luciferase (C1498-Luc) for 12 h. Representative flow cytometry (left) and statistics (right) of the IFN-γ + (top) and TNF + (bottom) of ILC1s ( n = 6). c Ncr1 +/+ ILC1s and Ncr1 gfp/gfp ILC1s sorted from the liver of Ncr1 +/+ and Ncr1 gfp/gfp mice, respectively, were cultured with C1498-Luc AML cells for 12 h. ILC1s were loaded into the bottom chambers of a 96-well Transwell plate. The top wells of the plate were loaded with C1498-Luc AML cells in the presence of IL-12 plus IL-15 <t>cytokines.</t> Statistics of the IFN-γ + (left) and TNF + (right) of ILC1s ( n = 4). Created in BioRender. Ma, R. (2024) https://BioRender.com/a44t133 . d Ncr1 +/+ ILC1s and Ncr1 gfp/gfp ILC1s sorted from the liver of Ncr1 +/+ and Ncr1 gfp/gfp mice, respectively, were cultured at the indicated ratios with C1498-Luc AML cells for 24 h. Luciferase activity in the wells with tumor cells was measured with a luminescence microplate reader ( n = 6). Created in BioRender. Ma, R. (2024) https://BioRender.com/n74x942 . Data were presented as means ± s.d. P values were calculated by either an unpaired Student’s t -test ( a – c ) or two-way ANOVA models with adjustments ( d ). NS not significant. Source data are provided as a Source Data file.
All Cytokines, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "NKp46 enhances type 1 innate lymphoid cell proliferation and function and anti-acute myeloid leukemia activity"

Article Title: NKp46 enhances type 1 innate lymphoid cell proliferation and function and anti-acute myeloid leukemia activity

Journal: Nature Communications

doi: 10.1038/s41467-025-55923-w

a Ncr1 +/+ ILC1s and Ncr1 gfp/gfp ILC1s sorted from the liver of Ncr1 +/+ and Ncr1 gfp/gfp mice, respectively, were cultured with or without anti-NKp46 antibody (5 μg/ml) for 12 h. Representative flow cytometry (left) and statistics (right) of the IFN-γ + (top) and TNF + (bottom) of ILC1s ( n = 6). b Ncr1 +/+ ILC1s and Ncr1 gfp/gfp ILC1s sorted from the liver of Ncr1 +/+ and Ncr1 gfp/gfp mice, respectively, were cultured with or without C1498 AML cells expressing luciferase (C1498-Luc) for 12 h. Representative flow cytometry (left) and statistics (right) of the IFN-γ + (top) and TNF + (bottom) of ILC1s ( n = 6). c Ncr1 +/+ ILC1s and Ncr1 gfp/gfp ILC1s sorted from the liver of Ncr1 +/+ and Ncr1 gfp/gfp mice, respectively, were cultured with C1498-Luc AML cells for 12 h. ILC1s were loaded into the bottom chambers of a 96-well Transwell plate. The top wells of the plate were loaded with C1498-Luc AML cells in the presence of IL-12 plus IL-15 cytokines. Statistics of the IFN-γ + (left) and TNF + (right) of ILC1s ( n = 4). Created in BioRender. Ma, R. (2024) https://BioRender.com/a44t133 . d Ncr1 +/+ ILC1s and Ncr1 gfp/gfp ILC1s sorted from the liver of Ncr1 +/+ and Ncr1 gfp/gfp mice, respectively, were cultured at the indicated ratios with C1498-Luc AML cells for 24 h. Luciferase activity in the wells with tumor cells was measured with a luminescence microplate reader ( n = 6). Created in BioRender. Ma, R. (2024) https://BioRender.com/n74x942 . Data were presented as means ± s.d. P values were calculated by either an unpaired Student’s t -test ( a – c ) or two-way ANOVA models with adjustments ( d ). NS not significant. Source data are provided as a Source Data file.
Figure Legend Snippet: a Ncr1 +/+ ILC1s and Ncr1 gfp/gfp ILC1s sorted from the liver of Ncr1 +/+ and Ncr1 gfp/gfp mice, respectively, were cultured with or without anti-NKp46 antibody (5 μg/ml) for 12 h. Representative flow cytometry (left) and statistics (right) of the IFN-γ + (top) and TNF + (bottom) of ILC1s ( n = 6). b Ncr1 +/+ ILC1s and Ncr1 gfp/gfp ILC1s sorted from the liver of Ncr1 +/+ and Ncr1 gfp/gfp mice, respectively, were cultured with or without C1498 AML cells expressing luciferase (C1498-Luc) for 12 h. Representative flow cytometry (left) and statistics (right) of the IFN-γ + (top) and TNF + (bottom) of ILC1s ( n = 6). c Ncr1 +/+ ILC1s and Ncr1 gfp/gfp ILC1s sorted from the liver of Ncr1 +/+ and Ncr1 gfp/gfp mice, respectively, were cultured with C1498-Luc AML cells for 12 h. ILC1s were loaded into the bottom chambers of a 96-well Transwell plate. The top wells of the plate were loaded with C1498-Luc AML cells in the presence of IL-12 plus IL-15 cytokines. Statistics of the IFN-γ + (left) and TNF + (right) of ILC1s ( n = 4). Created in BioRender. Ma, R. (2024) https://BioRender.com/a44t133 . d Ncr1 +/+ ILC1s and Ncr1 gfp/gfp ILC1s sorted from the liver of Ncr1 +/+ and Ncr1 gfp/gfp mice, respectively, were cultured at the indicated ratios with C1498-Luc AML cells for 24 h. Luciferase activity in the wells with tumor cells was measured with a luminescence microplate reader ( n = 6). Created in BioRender. Ma, R. (2024) https://BioRender.com/n74x942 . Data were presented as means ± s.d. P values were calculated by either an unpaired Student’s t -test ( a – c ) or two-way ANOVA models with adjustments ( d ). NS not significant. Source data are provided as a Source Data file.

Techniques Used: Cell Culture, Flow Cytometry, Expressing, Luciferase, Activity Assay



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a Ncr1 +/+ ILC1s and Ncr1 gfp/gfp ILC1s sorted from the liver of Ncr1 +/+ and Ncr1 gfp/gfp mice, respectively, were cultured with or without anti-NKp46 antibody (5 μg/ml) for 12 h. Representative flow cytometry (left) and statistics (right) of the IFN-γ + (top) and TNF + (bottom) of ILC1s ( n = 6). b Ncr1 +/+ ILC1s and Ncr1 gfp/gfp ILC1s sorted from the liver of Ncr1 +/+ and Ncr1 gfp/gfp mice, respectively, were cultured with or without C1498 AML cells expressing luciferase (C1498-Luc) for 12 h. Representative flow cytometry (left) and statistics (right) of the IFN-γ + (top) and TNF + (bottom) of ILC1s ( n = 6). c Ncr1 +/+ ILC1s and Ncr1 gfp/gfp ILC1s sorted from the liver of Ncr1 +/+ and Ncr1 gfp/gfp mice, respectively, were cultured with C1498-Luc AML cells for 12 h. ILC1s were loaded into the bottom chambers of a 96-well Transwell plate. The top wells of the plate were loaded with C1498-Luc AML cells in the presence of IL-12 plus IL-15 <t>cytokines.</t> Statistics of the IFN-γ + (left) and TNF + (right) of ILC1s ( n = 4). Created in BioRender. Ma, R. (2024) https://BioRender.com/a44t133 . d Ncr1 +/+ ILC1s and Ncr1 gfp/gfp ILC1s sorted from the liver of Ncr1 +/+ and Ncr1 gfp/gfp mice, respectively, were cultured at the indicated ratios with C1498-Luc AML cells for 24 h. Luciferase activity in the wells with tumor cells was measured with a luminescence microplate reader ( n = 6). Created in BioRender. Ma, R. (2024) https://BioRender.com/n74x942 . Data were presented as means ± s.d. P values were calculated by either an unpaired Student’s t -test ( a – c ) or two-way ANOVA models with adjustments ( d ). NS not significant. Source data are provided as a Source Data file.
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a Ncr1 +/+ ILC1s and Ncr1 gfp/gfp ILC1s sorted from the liver of Ncr1 +/+ and Ncr1 gfp/gfp mice, respectively, were cultured with or without anti-NKp46 antibody (5 μg/ml) for 12 h. Representative flow cytometry (left) and statistics (right) of the IFN-γ + (top) and TNF + (bottom) of ILC1s ( n = 6). b Ncr1 +/+ ILC1s and Ncr1 gfp/gfp ILC1s sorted from the liver of Ncr1 +/+ and Ncr1 gfp/gfp mice, respectively, were cultured with or without C1498 AML cells expressing luciferase (C1498-Luc) for 12 h. Representative flow cytometry (left) and statistics (right) of the IFN-γ + (top) and TNF + (bottom) of ILC1s ( n = 6). c Ncr1 +/+ ILC1s and Ncr1 gfp/gfp ILC1s sorted from the liver of Ncr1 +/+ and Ncr1 gfp/gfp mice, respectively, were cultured with C1498-Luc AML cells for 12 h. ILC1s were loaded into the bottom chambers of a 96-well Transwell plate. The top wells of the plate were loaded with C1498-Luc AML cells in the presence of IL-12 plus IL-15 <t>cytokines.</t> Statistics of the IFN-γ + (left) and TNF + (right) of ILC1s ( n = 4). Created in BioRender. Ma, R. (2024) https://BioRender.com/a44t133 . d Ncr1 +/+ ILC1s and Ncr1 gfp/gfp ILC1s sorted from the liver of Ncr1 +/+ and Ncr1 gfp/gfp mice, respectively, were cultured at the indicated ratios with C1498-Luc AML cells for 24 h. Luciferase activity in the wells with tumor cells was measured with a luminescence microplate reader ( n = 6). Created in BioRender. Ma, R. (2024) https://BioRender.com/n74x942 . Data were presented as means ± s.d. P values were calculated by either an unpaired Student’s t -test ( a – c ) or two-way ANOVA models with adjustments ( d ). NS not significant. Source data are provided as a Source Data file.
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a Ncr1 +/+ ILC1s and Ncr1 gfp/gfp ILC1s sorted from the liver of Ncr1 +/+ and Ncr1 gfp/gfp mice, respectively, were cultured with or without anti-NKp46 antibody (5 μg/ml) for 12 h. Representative flow cytometry (left) and statistics (right) of the IFN-γ + (top) and TNF + (bottom) of ILC1s ( n = 6). b Ncr1 +/+ ILC1s and Ncr1 gfp/gfp ILC1s sorted from the liver of Ncr1 +/+ and Ncr1 gfp/gfp mice, respectively, were cultured with or without C1498 AML cells expressing luciferase (C1498-Luc) for 12 h. Representative flow cytometry (left) and statistics (right) of the IFN-γ + (top) and TNF + (bottom) of ILC1s ( n = 6). c Ncr1 +/+ ILC1s and Ncr1 gfp/gfp ILC1s sorted from the liver of Ncr1 +/+ and Ncr1 gfp/gfp mice, respectively, were cultured with C1498-Luc AML cells for 12 h. ILC1s were loaded into the bottom chambers of a 96-well Transwell plate. The top wells of the plate were loaded with C1498-Luc AML cells in the presence of IL-12 plus IL-15 <t>cytokines.</t> Statistics of the IFN-γ + (left) and TNF + (right) of ILC1s ( n = 4). Created in BioRender. Ma, R. (2024) https://BioRender.com/a44t133 . d Ncr1 +/+ ILC1s and Ncr1 gfp/gfp ILC1s sorted from the liver of Ncr1 +/+ and Ncr1 gfp/gfp mice, respectively, were cultured at the indicated ratios with C1498-Luc AML cells for 24 h. Luciferase activity in the wells with tumor cells was measured with a luminescence microplate reader ( n = 6). Created in BioRender. Ma, R. (2024) https://BioRender.com/n74x942 . Data were presented as means ± s.d. P values were calculated by either an unpaired Student’s t -test ( a – c ) or two-way ANOVA models with adjustments ( d ). NS not significant. Source data are provided as a Source Data file.
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a Ncr1 +/+ ILC1s and Ncr1 gfp/gfp ILC1s sorted from the liver of Ncr1 +/+ and Ncr1 gfp/gfp mice, respectively, were cultured with or without anti-NKp46 antibody (5 μg/ml) for 12 h. Representative flow cytometry (left) and statistics (right) of the IFN-γ + (top) and TNF + (bottom) of ILC1s ( n = 6). b Ncr1 +/+ ILC1s and Ncr1 gfp/gfp ILC1s sorted from the liver of Ncr1 +/+ and Ncr1 gfp/gfp mice, respectively, were cultured with or without C1498 AML cells expressing luciferase (C1498-Luc) for 12 h. Representative flow cytometry (left) and statistics (right) of the IFN-γ + (top) and TNF + (bottom) of ILC1s ( n = 6). c Ncr1 +/+ ILC1s and Ncr1 gfp/gfp ILC1s sorted from the liver of Ncr1 +/+ and Ncr1 gfp/gfp mice, respectively, were cultured with C1498-Luc AML cells for 12 h. ILC1s were loaded into the bottom chambers of a 96-well Transwell plate. The top wells of the plate were loaded with C1498-Luc AML cells in the presence of IL-12 plus IL-15 <t>cytokines.</t> Statistics of the IFN-γ + (left) and TNF + (right) of ILC1s ( n = 4). Created in BioRender. Ma, R. (2024) https://BioRender.com/a44t133 . d Ncr1 +/+ ILC1s and Ncr1 gfp/gfp ILC1s sorted from the liver of Ncr1 +/+ and Ncr1 gfp/gfp mice, respectively, were cultured at the indicated ratios with C1498-Luc AML cells for 24 h. Luciferase activity in the wells with tumor cells was measured with a luminescence microplate reader ( n = 6). Created in BioRender. Ma, R. (2024) https://BioRender.com/n74x942 . Data were presented as means ± s.d. P values were calculated by either an unpaired Student’s t -test ( a – c ) or two-way ANOVA models with adjustments ( d ). NS not significant. Source data are provided as a Source Data file.
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a Ncr1 +/+ ILC1s and Ncr1 gfp/gfp ILC1s sorted from the liver of Ncr1 +/+ and Ncr1 gfp/gfp mice, respectively, were cultured with or without anti-NKp46 antibody (5 μg/ml) for 12 h. Representative flow cytometry (left) and statistics (right) of the IFN-γ + (top) and TNF + (bottom) of ILC1s ( n = 6). b Ncr1 +/+ ILC1s and Ncr1 gfp/gfp ILC1s sorted from the liver of Ncr1 +/+ and Ncr1 gfp/gfp mice, respectively, were cultured with or without C1498 AML cells expressing luciferase (C1498-Luc) for 12 h. Representative flow cytometry (left) and statistics (right) of the IFN-γ + (top) and TNF + (bottom) of ILC1s ( n = 6). c Ncr1 +/+ ILC1s and Ncr1 gfp/gfp ILC1s sorted from the liver of Ncr1 +/+ and Ncr1 gfp/gfp mice, respectively, were cultured with C1498-Luc AML cells for 12 h. ILC1s were loaded into the bottom chambers of a 96-well Transwell plate. The top wells of the plate were loaded with C1498-Luc AML cells in the presence of IL-12 plus IL-15 <t>cytokines.</t> Statistics of the IFN-γ + (left) and TNF + (right) of ILC1s ( n = 4). Created in BioRender. Ma, R. (2024) https://BioRender.com/a44t133 . d Ncr1 +/+ ILC1s and Ncr1 gfp/gfp ILC1s sorted from the liver of Ncr1 +/+ and Ncr1 gfp/gfp mice, respectively, were cultured at the indicated ratios with C1498-Luc AML cells for 24 h. Luciferase activity in the wells with tumor cells was measured with a luminescence microplate reader ( n = 6). Created in BioRender. Ma, R. (2024) https://BioRender.com/n74x942 . Data were presented as means ± s.d. P values were calculated by either an unpaired Student’s t -test ( a – c ) or two-way ANOVA models with adjustments ( d ). NS not significant. Source data are provided as a Source Data file.
All Cytokines Were Purchased From, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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a Ncr1 +/+ ILC1s and Ncr1 gfp/gfp ILC1s sorted from the liver of Ncr1 +/+ and Ncr1 gfp/gfp mice, respectively, were cultured with or without anti-NKp46 antibody (5 μg/ml) for 12 h. Representative flow cytometry (left) and statistics (right) of the IFN-γ + (top) and TNF + (bottom) of ILC1s ( n = 6). b Ncr1 +/+ ILC1s and Ncr1 gfp/gfp ILC1s sorted from the liver of Ncr1 +/+ and Ncr1 gfp/gfp mice, respectively, were cultured with or without C1498 AML cells expressing luciferase (C1498-Luc) for 12 h. Representative flow cytometry (left) and statistics (right) of the IFN-γ + (top) and TNF + (bottom) of ILC1s ( n = 6). c Ncr1 +/+ ILC1s and Ncr1 gfp/gfp ILC1s sorted from the liver of Ncr1 +/+ and Ncr1 gfp/gfp mice, respectively, were cultured with C1498-Luc AML cells for 12 h. ILC1s were loaded into the bottom chambers of a 96-well Transwell plate. The top wells of the plate were loaded with C1498-Luc AML cells in the presence of IL-12 plus IL-15 cytokines. Statistics of the IFN-γ + (left) and TNF + (right) of ILC1s ( n = 4). Created in BioRender. Ma, R. (2024) https://BioRender.com/a44t133 . d Ncr1 +/+ ILC1s and Ncr1 gfp/gfp ILC1s sorted from the liver of Ncr1 +/+ and Ncr1 gfp/gfp mice, respectively, were cultured at the indicated ratios with C1498-Luc AML cells for 24 h. Luciferase activity in the wells with tumor cells was measured with a luminescence microplate reader ( n = 6). Created in BioRender. Ma, R. (2024) https://BioRender.com/n74x942 . Data were presented as means ± s.d. P values were calculated by either an unpaired Student’s t -test ( a – c ) or two-way ANOVA models with adjustments ( d ). NS not significant. Source data are provided as a Source Data file.

Journal: Nature Communications

Article Title: NKp46 enhances type 1 innate lymphoid cell proliferation and function and anti-acute myeloid leukemia activity

doi: 10.1038/s41467-025-55923-w

Figure Lengend Snippet: a Ncr1 +/+ ILC1s and Ncr1 gfp/gfp ILC1s sorted from the liver of Ncr1 +/+ and Ncr1 gfp/gfp mice, respectively, were cultured with or without anti-NKp46 antibody (5 μg/ml) for 12 h. Representative flow cytometry (left) and statistics (right) of the IFN-γ + (top) and TNF + (bottom) of ILC1s ( n = 6). b Ncr1 +/+ ILC1s and Ncr1 gfp/gfp ILC1s sorted from the liver of Ncr1 +/+ and Ncr1 gfp/gfp mice, respectively, were cultured with or without C1498 AML cells expressing luciferase (C1498-Luc) for 12 h. Representative flow cytometry (left) and statistics (right) of the IFN-γ + (top) and TNF + (bottom) of ILC1s ( n = 6). c Ncr1 +/+ ILC1s and Ncr1 gfp/gfp ILC1s sorted from the liver of Ncr1 +/+ and Ncr1 gfp/gfp mice, respectively, were cultured with C1498-Luc AML cells for 12 h. ILC1s were loaded into the bottom chambers of a 96-well Transwell plate. The top wells of the plate were loaded with C1498-Luc AML cells in the presence of IL-12 plus IL-15 cytokines. Statistics of the IFN-γ + (left) and TNF + (right) of ILC1s ( n = 4). Created in BioRender. Ma, R. (2024) https://BioRender.com/a44t133 . d Ncr1 +/+ ILC1s and Ncr1 gfp/gfp ILC1s sorted from the liver of Ncr1 +/+ and Ncr1 gfp/gfp mice, respectively, were cultured at the indicated ratios with C1498-Luc AML cells for 24 h. Luciferase activity in the wells with tumor cells was measured with a luminescence microplate reader ( n = 6). Created in BioRender. Ma, R. (2024) https://BioRender.com/n74x942 . Data were presented as means ± s.d. P values were calculated by either an unpaired Student’s t -test ( a – c ) or two-way ANOVA models with adjustments ( d ). NS not significant. Source data are provided as a Source Data file.

Article Snippet: All cytokines were from Peprotech or the National Institutes of Health.

Techniques: Cell Culture, Flow Cytometry, Expressing, Luciferase, Activity Assay